Purification and structure elucidation of paraplantaricin TC318, a new lantibiotic produced by Lactobacillus paraplantarum OSY-TC318
Loading...
Date
2019-03
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
A new Lactobacillus paraplantarum strain isolated from a Turkish cheese showed potent antimicrobial activity that inhibits Bacillus cereus, Micrococcus luteus, Lactobacillus casei and Pediococcus pentosaceus. The producer strain, designated Lactobacillus paraplantarum OSY-TC318, was identified using rapid MALDI-TOF mass spectrometry (MS), 16S rDNA sequencing and further confirmed by whole genome sequencing (WGS). The antimicrobial agent was extracted from cells of OSY-TC318 with 70% isopropanol, purified by high-performance liquid chromatography, and structurally analyzed using Fourier Transform Ion Cyclotron Resonance (FT-ICR) mass spectrometry. The MS and WGS analyses, taken together, elucidated the structure of the novel lantibiotic consisting of 22 amino acids and four thioether bridges. The molecular mass of the new lantibiotic, designated paraplantaricin TC318, is 2263.900 Da. Paraplantaricin TC318 contains one didehydroalanine (Dha), one didehydrobutyric acid (Dhb), one methyllanthionine (MeLan), two lanthionine (Lan) residues and a C-terminal S-[(Z)-2-aminovinyl]-D-cysteine (AviCys) residue. The proposed primary sequence of paraplantaricin TC318 is: F-K-A-W-Dha-L-A-Abu-F-G-A-G-H-Dhb-G-A-F-N-A-F-A. This lantibiotic, which differs from mutacin 1140 at positions 9, 12, 13 and 20, is a new member of the epidermin group in class I lantibiotic.
Description
Poster Division: Health Sciences: 1st Place (The Ohio State University Edward F. Hayes Graduate Research Forum)
Keywords
bacteriocin, lantibiotic, Lactobacillus paraplantarum, paraplantaricin TC318