Knowledge Bank

Observations here reported were made in connection with isolation chromosome studies in the Laboratory of Colloidal Chemistry, Ohio State $University.1$ Material was subjected to study under light and electron microscopes. Masses of isolated chromosomes from bronze turkey erythrocytes (TEC) were treated according to the Bretschneider staining procedure, with some $modifications.1,2,3,4$ At the end of this procedure, step No. 6, after completion of washing, to residues in the tubes was added 0.1N HCl and the tubes were returned to a $40\circ C$ water bath. From tubes of chromosomes (TEC), and from washed nuclei (TEN) given the same treatment, there developed linear structures from the masses of stained material with spherical nodules at the outgoing end, usually with a bright red center. These linear structures were subjected to various technical methods to determine solubility, structure of molecules, etc. They were most sensitive to every thermal and light change, making photographing in the resonance field under the light microscope impossible. From a particular lot of blood collected 4-24-53 from breeding male bronze turkeys, definite outstanding observations were made as to colors of these structures--which were dark blue, yellow, and pink. When these linear structures were placed under the microscope, the dark blue strands were observed to segment (5-8-53, 5:30 p.m.). A second slide did likewise in 30 minutes. Remaining residual strands were then washed in distilled water, and a third slide, strands in water, placed under the microscope. In 30 minutes the dark blue and some of the yellow and pink strands had segmented. Other tubes of linear strands plus $28$ (three different lots), left in a rack at room temperature ($20\circ -23\circ C$), with fluorescent lighting, appeared to live, grow, and multiply through a period of three months. Fungal, nematodal, and virus structures from the same tube indicate that a synthesis (thermo-electromagnetic) to these structures from material of chromosomal origin has taken place. In a resonance field under the microscope with no structures, there were suddenly one, two, or more bright line structures, which then grew, acquired thickness and length, and segmented. Hydrolyzate of TEC, by fifteen chromatogram charts, indicates the presence of nine to eleven amino acids.