The role of Cannabidiol in inhibiting tumorigenesis in cisplatin resistant Non-Small Cell Lung Cancer
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Date
2022-04
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Abstract
Lung Cancer is one of the deadliest cancers known and has less than a 20% survival rate. This is due to lung cancer having the ability to have its cancerous cells multiply rapidly and metastasize. One of the notable types of lung cancer is Non-Small Cell Lung Cancer (NSCLC). Patients with NSCLC are often treated with cisplatin as the first line of therapy due to tumor resections' structural and location complexities. However, cisplatin has been deemed not so effective due to the high drug-resistant rate due to the expansion of cancer stem cells (CSCs). This CSCs have self-renewing properties and the ability to systematically divide. Cannabidiol (CBD), which is a non-psychoactive phytochemical derived from the Cannabis plant, has been identified as a potential treatment against cisplatin-resistant NSCLC cells. In this project, there were various amounts os methods used to analyze the relationship between CBD treatment and NSCLC. This project utilized H460 (large cell carcinoma) and A5499 (adenocarcinoma) cells by using H460 and A549-parental (PT) and cisplatin-resistant CR cells. Initially, cell viability assays using Presto Blue dye were utilized to determine the number of cells that will be present after the parental and cisplatin-resistant cell lines. Then we used APC-labeled Annexin-V and AAD for apoptotic analysis by staining the drug-resistant H460-CR and A549-CR NSCLC cells. Western Blots were used to establish the total concentration of the apoptotic event. Finally, animal studies compared the tumor dimensions of NSCLC cells treated with cisplatin, CBD, or control (PBS). The data exhibited that the cell viability of the CBD-treated CR NSCLC cells was lower in both cell lines (H460, A549) compared to the NSCLC parental cells (H460-PT, A549-PT). It was indicated that the reduced viability in CBD-treated CR NSCLC cells was linked to increased apoptosis by observing the expression of the apoptotic markers, cleaved caspases 3 and 9 using Western Blots. It has been noted that CBD treatment reduces tumor progression and metastasis but also suppresses cancer stem cell properties. Our results demonstrate great promise about the positive impact of CBD treatment on Non-Small Cell Lung Cancer.
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Non-Small Cell Lung Cancer, Cancer, Cisplatin, Cannabidiol