Synthesis of a pH Sensitive Injectable Hydrogel for Effective Transplantation of Cardiosphere Derived Stem Cells to Support Myocardial Remodeling
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Date
2013-12
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Publisher
The Ohio State University
Abstract
Myocardial infarction (MI), commonly known as a heart attack, is the leading cause of death in the United States. Myocardial damage often results in chronic heart failure due to an insufficient regeneration of cardiomyocytes. Much of the developments used to sufficiently regenerate the damaged tissue center around implanting cardiosphere derived stem cells (CDCs) into the heart. This study focuses on developing a hydrogel matrix with the necessary mechanical, thermal, and biological properties to allow for proper delivery of CDCs into the damaged region. The methods used to study various properties were 1H-NMR for composition, GPC for molecular weight determination, DSC for polymer lower critical solution temperature, parallel plate rheometry and tensile test for modulus, a catheter injection for injectability and CDCs encapsulation for biocompatibility. A series of dual pH- and thermo-sensitive hydrogel was developed by free radical copolymerization of N-isopropyl acrylamide, propylacrylic acid, hydroxyehtyl methacrylate-co-polytrimethylene. One of the hydrogels showed optimal mechanical behaviors and biocompatibility, which makes it a promising cell delivery carrier.
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Keywords
Hydrogel, pH sensitive, Thermo-responsive, synthesis