In vitro Anti-Tumor Efficacy of Trimeric in Head and Neck Cancer
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Date
2015-05
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The Ohio State University
Abstract
In this project, we will determine the anti-tumor activity of trimeric in head and neck squamous cell carcinoma (HNSCC) cells. Trimeric is a novel formulation consisting of three natural products with known anti-cancer activity. The goal of the study was to assess the efficacy of trimeric, as a standard of chemotherapeutic care, to inhibit the viability of HNSCC in vitro. Two established HNSCC cell lines, CAL27 and UMSCC-47, and non-tumorigenic human keratinocytes (HaCaT) were treated with trimeric for 24-72 h and dose response curves were generated. The IC50 values for each treatment regimen for HNSCC cells and HaCaT cells were calculated and compared to determine the therapeutic index. It was determined that trimeric showed chemotherapeutic activity in all three cell lines over both 24 and 48 h treatment regimens. In addition, anti-cancer activity was determined in both HNSCC cell lines in the 72 h trial. No significance was found in differential responses between cell lines. We explored the mechanism of action of trimeric with a focus on promotion of cell toxicity. Annexin V-positive apoptotic CAL27 cells substantiated that trimeric induced apoptosis in this HPV-negative cell line.
This proposal has several implications to the field of head and neck squamous cell carcinoma (HNSCC). Our results provide key insight to the efficacy and mechanism of action of trimeric, a novel formulation of three natural products, on HNSCC. These are necessary initial steps for the development of trimeric as a potential anti-cancer therapeutic to manage HNSCC patients.
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Keywords
head and neck cancer, trimeric, squamous cell carcinoma, anti-cancer, chemotherapeutic