Bone marrow-derived stromal cells and Notch ligands support the sequential acquisition of CD94 and NKp80 during human NK cell development

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2019-03

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Research Projects

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Abstract

Human natural killer (NK) cells develop in secondary lymphoid tissues (SLTs) from precursor cells that differentiate into NK and other innate lymphoid cell (ILC) subsets. We investigated the role of Notch signaling in NK cell maturation. We used a stromal feeder cell development assay together with activation of Notch signaling to generate mature NK cells in vitro from SLT-derived ILC precursor cells (ILCPs). NK cell developmental stages express Notch receptors at the surface. Activation of Notch in immature NK cells promoted the developmental transition wherein NK cells attain functional maturity. In vitro-derived NK cells in the presence of Notch activation expressed the surface activating receptor NKp80, expressed transcription factors T-BET and EOMES, and produced perforin, granzymes, and interferon-γ. These data identify Notch signaling as a cellular pathway regulating NK cell functional maturation.

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Health Sciences: 2nd Place (The Ohio State University Edward F. Hayes Graduate Research Forum)

Keywords

immunology, NK cells, innate immunity, Notch signaling

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