OBSERVATION AND ANALYSIS OF SERS SPECTRUM OF LYSOZYME ON SILVER COLLOID
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Date
1992
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Ohio State University
Abstract
The SERS spectrum of lysozyme on silver colloid was obtained. The sample solution was prepared by mixing O-2 ml silver colloid with 0.2 ml lysozyme. In the solutionn, the concentration of lysozyme was 6$\times10^{-2}$TM, chloride (I) was about $1\times10^{-3}$M and PH was 2.0. In the spectrum, the bands at 356, 424, 660, 828, 928, 978, 1190 and $1592 cm^{-1}$ were assigned to the p-hydroxyphenyl ring vibrations of tyrosine. The bands at 424, 620, 928, 1190 and $1508 cm^{-1}$ were assigned to the vibrations of phenylalanine. The bands at 760, 928, 1358, $1562 cm^{-1}$ due to the vibrations of tryptophan. The band at $238 cm^{-1}$ was the interface vibration. The S-S bond peak was observed at $518 cm^{-1}$ and it was very weak. The amide I, II, III band were observed at 1646, 1528 and $1280 cm^{-1}$, respectively. The band at $928 cm^{-1}$ was the skeletal vibration ($C_{O}$-C-N stretching). These suggested that lysozyme molecules adsorbed on the colloidal silver particales with the part of $\alpha$-helix conformation. This also confined that, as same as the normal Raman spectroscopy, the SERS spectrum could be used to obtain the valuable information of the macromolecules.
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Author Institution: Department of Chemistry, Wuhan University; Center of Analysis and Measurement, Wuhan University; Department of Chemistry, Wuhan University