Enterovirus Plaque Technique : Utilization of Maintenance Medium on Agar Overlay without Neutral Red

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1966-09

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Abstract

A plaque technique for quantitating enteric viruses using liquid growth medium on the agar overlay was developed. Deletion of neutral red and staining of plaques using crystal violet, following plaque development, facilitated the technique. This method utilized a minimal number of monkey kidney epithelial cells for testing the greatest number of samples.

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Author Institution: The Defiance College, Defiance, Ohio

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The Ohio Journal of Science. v66 n5 (September, 1966), 523-527