THE PREPARATION OF SAMPLES IN THE SOLID STATE FOR SPECTROSCOPIC STUDY IN THE INFRARED AND ULTRAVIOLET REGIONS

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1951

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Ohio State University

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The desirability of correlating both the ultraviolet and infrared absorption for purposes of characterization and for theoretical consideration is recognized. However, the limitation imposed by the solubility of many organic solids makes uniform conditions for solution work over this range of the spectrum impossible. The further problem of solvent-sample interaction and of solvent absorption, where this is selective, cannot be overlooked. A method for readily preparing organic materials for spectrophotometric study in both the ultraviolet and infrared regions has been developed. The method involves the thorough mixing of the finely ground organic with solid potassium bromide and subsequent pressing into disks. The method to be described has been employed for study of the free base of guanine and also for the hydrochloride, and also has been used for anhydrous cytosine and for the crystalline hydrate. Other types of compounds also will be shown. The range of concentrations and disk thicknesses which will permit quantitative determination of intensity as expressed by molecular extinction coefficients will be discussed.

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Author Institution: Sienna Heights College, Adrian, Michigan

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