The Quantification of the Drug Gabapentin in Tissue Samples by HPLC-ESI-TOF-MS
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Date
2015-05
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The Ohio State University
Abstract
Gabapentin is being studied for use after high-level spinal cord injury (above thoracic level five) to prevent abnormal neuron sprouting and aberrant synaptogenesis which causes a life threatening condition Autonomic Dysreflexia (AD). Without treatment, AD causes high blood pressure which can induce stroke, seizures, and even death. It is theorized that gabapentin will reduce swelling around the site of injury which will enhance axon growth and plasticity culminating in improved recovery of neurological function after spinal cord injury. High performance liquid chromatography electrospray ionization time-of-flight mass spectroscopy (HPLC-ESI-TOF-MS) was used for the quantification of gabapentin in tissue serum samples. Solutions of samples were prepared and concentrations of drug were determined on the basis of calibration curves created using standards prepared. The samples were spiked with an internal standard directly after tissue harvest and prior to any further manipulation to correct for variations in sample preparation and analysis. This separation and detection method is suitable for quantitative sample analysis at low concentrations. With the developed method, it has been shown that HPLC-ESI-TOF-MS is capable of determining the concentrations of gabapentin in serums. This method of detection will assist neuroscientists in the determination of efficacious dosages as well as administration methods.
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Keywords
gabapentin, spinal cord injury, Autonomic Dysreflexia, High Performance Liquid Chromatography, Time of Flight- Mass Spectroscopy, detection method