Role of Arabidopsis PAS/LOV protein in stress response
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Date
2008-06
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Publisher
The Ohio State University
Abstract
Plant functioning and growth are strongly affected by dehydration and salt stress conditions. Many genes are involved in the response pathways that are activated to tolerate stress. It has been shown that Arabidopsis PAS/LOV protein (PLP) is transcriptionally upregulated under high salt and dehydration stress conditions. In order to investigate the role of PLP in stress response, ten homozygous knockout insertion lines were identified by PCR. Four weak overexpression lines were also identified using RT-PCR. PLP, which contains a LOV domain, did not exhibit an obvious circadian cycling phenotype at the transcriptional level. Germination success on high salt medium was impaired in the PLP knockout when compared to Wild Type. However, measurement of root growth on high salt medium did not reveal any additional phenotype for PLP knockout. Transcriptional expression of two known stress response genes, RD29A and DREB2A, showed differential expression when comparing Wild Type and PLP knockout plants under strong stress conditions. Under both high salt and dehydration stress conditions, RD29A and DREB2A exhibit reduced upregulation and shortened expression period when PLP is absent. These results indicate that PLP is likely involved in the response pathways for dehydration and salt stress.
Description
3rd place in Biology, Denman Undergraduate Research Forum 2008
Keywords
Arabidopsis, PAS/LOV, salt stress, dehydration stress