Tannic Acid Effects on Raphanus Raphanistrum Root Acid Phospatase
Loading...
Date
2000-12
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The effects of tannic acid (TA) on acid phosphatase (APase) (E.C.3.1.3-2) from root extracts of fertilized or unfertilized Raphanus raphanistrum plants were examined. TA inhibited APase activity non-competitively; Vmax was reduced, but the apparent K^ was unaffected in the presence of added TA. Endogenous tannin content ranged between 0.26 and 0.56 (Imoleg fresh weight * of root tissue. Enzyme activity data was extrapolated to provide estimates of activity in the absence of any TA; uncorrected APase activity of R. raphanistrum was underestimated by an average of 25%. Addition of polyvinylpyrrolidone (PVP) did not affect measured enzyme rates in a consistent manner.
Description
Author Institution: Department of Biology and Department of Chemistry, Hiram College
Keywords
Citation
The Ohio Journal of Science. v100, n5 (December, 2000), 132-135