A Rapid Technique for the Detection of Nicotine in Developing Tobacco Seedlings

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V073N6_353.pdf (315.84 KB)

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1973-11

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Abstract

A rapid and sensitive method for detecting alkaloids, in particular nicotine, from Nicotiana rustica tobacco seedlings up to 2 mm in length has been developed. Growing tissue is applied (squashed) directly onto silica gel plates for thin-layer chromotographic analysis. The sensitivity of this method permits the detection of quantities of nicotine as small as 0.4 microgram.

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Author Institution: Department of Botany and Institute of Polar Studies and Department of Microbiology, The Ohio State University

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The Ohio Journal of Science. v73, n6 (November, 1973), 353-356

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http://hdl.handle.net/1811/22186

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Ohio Journal of Science: Volume 73, Issue 6 (November, 1973)