Taggable Pyrrolysine Analogs

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McCormickKathleen_Thesis.pdf (6.91 MB)

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2009-06

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The Ohio State University

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Abstract

N6-(1,3-dioxolancarbonyl)lysine was synthesized and incorporated into full-length mCherry and alpha synuclein proteins by reading through a UAG codon. Mass spectrometry analysis confirmed that the analog incoporates into alpha synuclein in the acetal form. Two additional pyrrolysine analogs, N6-(2-oxo-2H-chromene-3-oyl)lysine trifluoroacetate and N6-(aminoxyacetoyl)lysine ditrifluoroacetate, were synthesized but neither compound reads throuh the UAG codon.

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pyrrolysine, analogs

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http://hdl.handle.net/1811/63966

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Arts and Sciences Undergraduate Research Theses and Honors Research Theses