Correlation Between SRID and ELISA of Serum Immunoglobulin G Concentrations in Quarter Horse Mares and Their Foals From Birth to Weaning
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Date
2015-05
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The Ohio State University
Abstract
Failure of passive transfer (FPT) is a life threatening condition in foals and occurs when immunoglobulin G concentrations (IgG) are below 400 mg/dL and partial failure of passive transfer (PFPT) occurs when IgG concentrations are between 400-800 mg/dL. An IgG concentration of greater than 800 mg/dL is considered sufficient to protect the foal against pathogens. The standard method of determining antibody concentrations is Single Radial Immunodiffusion (SRID). However, this method requires an 18 h incubation and is not time efficient. Enzyme Linked Immunosorbant Assays (ELISA) only require a 4 h incubation period to detect IgG concentrations. The purpose of this study is the correlate the immunoglobulin G concentrations of mares and foals derived from ELISA with a previous study conducted using SRID. A low correlation between ELISA and SRID was found and within blocks, foals displayed the highest correlations.
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Keywords
Immunoglobulins, Antibodies, Horse, ELISA, SRID