A quantitative exploration of wounding-induced changes to tomato steroidal glycoalkaloid profiles in diverse tomato fruits
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Date
2022-12
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The Ohio State University
Abstract
It is well documented that mechanical wounding has the capability to produce a variety of local and systemic effects in plants, including alterations to their phytochemical profiles. Tomato steroidal glycoalkaloids (tSGAs) are a class of insecticidal and fungicidal cholesterol-derived metabolites produced by members of the tomato clade. Mass spectrometry-based imaging techniques such as MALDI-MSI have elucidated qualitative changes in metabolite composition at the site of wounding in tomato fruit; however, these results lack quantitative details and do not provide insight into changes on a whole-fruit scale. Additionally, these studies have evaluated post-harvest fruit from a limited range of cultivars over relatively short periods of time. To contribute a quantitative analysis of wounding-induced phytochemical profile changes on a whole-fruit scale, factor in on-plant maturation, and examine the responses across cultivars, this study examined the tSGA profiles of wounded tomato fruits across four accessions via ultra-high performance liquid chromatography, tandem mass spectrometry (LC-MS/MS). Four diverse accessions of tomato were selected –Tainan, LA2522, OH8243, and LA2213–due to their differing levels of baseline alkaloid content and varying degrees of cultivation. Fruits were wounded on-plant at the mature green and mature red maturation stages to provide insight into the endurance and intensity of any potential changes to tSGA profiles. Overall, this study explores the inducibility of tomato steroidal glycoalkaloid profile changes and further illuminates tomato wounding responses throughout the ripening process and across diverse accessions.
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Keywords
phytochemicals, tomato steroidal glycoalkaloids, mass spectrometry, host defense, plant science, phytochemistry