Inhibition of cell migration and endothelial cell tubulogenesis by compounds found in black raspberries.
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Date
2007-08
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The Ohio State University
Abstract
Cell migration and endothelial cell tubulogenesis are both processes which are altered in many cancers. Black raspberries and their components have been shown to inhibit tumor growth and neovascularization in vivo, but the active components in black raspberries are still being identified. This experiment used two assays to determine the effect of black raspberry components on tumor cell migration, endothelial cell migration, and endothelial cell tubulogenesis. Ellagic acid and quercetin effectively inhibited migration of both cell types. Human Umbilical Vascular Endothelial Cell (HUVEC) migration was inhibited by ellagic acid at 2 µM (30.9% control ± 10.1%, P ≤ 0.04), and by quercetin at 10 µM (24.1% control ± 2.5%, P ≤ 0.02). Migration of the esophageal tumor cell line (TE-8) was studied to evaluate differences between endothelial cell and tumor cell sensitivity to each compound. Migration of TE-8 cells was inhibited by ellagic acid at 2 µM (43.1% control ± 15.9%, P ≤ 0.04), and by quercetin at 20 µM (40.3% control ± 18.7%, P ≤ 0.05). Both ellagic acid and quercetin inhibited endothelial cell tubulogenesis qualitatively at 2 µM. However, HUVEC morphology differed between the two treatments. Ellagic acid inhibited tubulogenesis and cell elongation at 2 µM, whereas quercetin inhibited tubulogenesis, but not cell elongation at 2 µM.
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Keywords
cancer, cell migration, angiogenesis, HUVEC