Dual Drug Release from CO2-Infused Nanofibers via Hydrophobic and Hydrophilic Interactions
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Date
2014-05
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The Ohio State University
Abstract
CO2 infusion of biomolecules is a benign, green, and inexpensive method to provide much-needed biochemical activity to electrospun nanofibers for tissue engineering applications. This study investigated the effects of hydrophobic-hydrophilic interactions on dual drug release from CO2-infused nanofibers (PCL, PCL-gelatin, and PCL ‘core’ PCL-gelatin ‘shell’) using BODIPY 493/503 and Rhodamine B fluorescent dyes as drug models. Interestingly, when exposed to supercritical CO2, core-shell fibers did not melt. Positive dye-matrix interactions led to increased dye loading and gradual, linear release. Conversely, the opposite was observed for negative interactions. When two dyes were infused, this behavior was accentuated due to interactions between dyes. CO2 infusion, without changing scaffold microstructure, positively impacted both dye loading and longer-term release when individual dyes were infused into scaffolds of unlike polarity. Core-shell nanofibers displayed radically different release properties versus PCL-gelatin when treated with dyes via CO2 infusion. Dye release from core-shell scaffolds was highly sensitive to both interactions with scaffolds and phase of CO2. By using different phases of CO2 to partition dyes into hydrophobic and hydrophilic sections of core-shell nanofiber scaffolds, interactions can be manipulated to develop a bimodal drug release system.
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Keywords
electrospinning, multi drug delivery, wound healing, supercritical CO2, core-shell, lipophilic-lipophobic