Optimizing Actuation of Assembly of DNA Origami Nano Structures
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Date
2021-05
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The Ohio State University
Abstract
DNA origami as an advanced nanotechnology has been useful in providing precise design and
construction for nanostructures. Combining with different actuation mechanisms such as toehold
mediated strand displacement (TMSD) or magnetic actuation can realize precise control in planar
or spatial motion for nanoscale structures. Recent efforts have expanded DNA origami to
micrometer scale nanostructures by integrating many nanocomponents into larger assemblies,
and a key goal of this work is to achieve real-time multiplexing actuation over these micronscale assemblies. Firstly, focusing on a stiff micro-scale DNA lever assembly, this work has
tested different assay conditions including varying concentrations, incubation time and
purification parameters to optimize the yield of individual DNA origami structure sub-units as a
basis for higher order lever assembly. Also this work aims to maximize yield of at least 1-2
micrometer polymerized nanostructures, which represents a key step for practical multiplexing.
Secondly, moving towards assemblies with complex reconfiguration capabilities, a verification
of reconfigurability and complex motion for a second structure system was conducted. The
second system consists of a DNA origami structure comprised of 6-bars connected into a closed
loop. The structure can be reconfigured into several different shapes including a rectangle,
triangle, hexagon, and flat closed shape. Broadly speaking, this work has integrated optimized
construction for a magnetic actuated nanopolymer and verification of the feasibility of
reconfiguration for a nanostructure with high order degree of freedoms. This work expands the
possibilities for complex design and practical construction at micrometer scale which enables
real-time control over DNA origami structures with complex reconfigurtion.
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Keywords
optimization for DNA origami assembly