Auxinic Herbicide Damage Quantification and Mitigation

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Date

2024-12

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The Ohio State University

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Abstract

In the U.S., the use of auxinic herbicides like 2,4-D and dicamba for weed control monoculture row crop systems continues to rise. The volatile nature of these chemicals and inappropriate application can lead to off-target drift, causing auxin transport misregulation and damage to broadleaf plants and woody species. Vitis vinifera (grape) is particularly vulnerable, as damage from auxinic herbicide drift can lead to stunted growth, yield loss, and vine death. The project aimed to develop methods to quantify herbicide residues on plant leaves, using novel technology developed by the Blakeslee Laboratory and QQQ LC-MS/MS analysis. Additionally, the project investigated potential pre-protectant substances that could be used to protect vineyards from herbicide drift damage. Experimentation involved growing grape cuttings hydroponically in a growth chamber and subjecting plants to simulated drift events, followed by developing methods to improve the efficiency of herbicide residue analysis using previously developed technology from the Blakeslee Lab.

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herbicide drift, auxinic herbicides, Vitis vinifera, QQQ LC-MS/MS, liquid chromatography-mass spectrometry, method development

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