Determining the Influence of Histone Tails on Compaction and Transcription Factor Binding

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2013-05

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The Ohio State University

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Abstract

Histone octamer tails play an important role in regulating transcription factor binding and nucleosome compaction. In order to determine the mechanisms by which the histone octamer tails play a role in such processes, the tails were labeled with a spin label, and tracked using electron paramagnetic resonance (EPR). Changes in tail mobility were quantified using EPR and to indicate histone octamer interaction with the DNA and/or with the histone octamer protein itself. EPR spectra of the H3 histone tails were compared before and during compaction. The results of the compaction study were inconclusive, leading to a change in the type of spin label.

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EPR, Nucleosomes, Histone tails, electron paramagnetic resonance, LexA, spin label

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http://hdl.handle.net/1811/54421

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Engineering Undergraduate Research Theses and Honors Research Theses