INFRARED SPECTROSCOPY IN THE FIELD OF $BACTERIOLOGY^{*}$
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Date
1957
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Ohio State University
Abstract
“This presentation brings up to date the results of a research project of which previous reports have been made at these meetings. In the beginning, the work was purely exploratory to see to what extent infrared spectroscopy could be used in biological research where complex mixtures were involved. The specific problem was concerned with the lipid mixtures extracted from the tubercle bacillus. It was demonstrated that with highly standardized procedures, reproducible results could be obtained. Certain strains of the tubercle bacillus were identified by the infrared spectra of some of these simpler mixtures. Column chromatography was adopted to obtain still simpler mixtures, and in some instances single compounds were identified in the eluates. The compounds thus isolated from the lipid extracts of the tubercle bacillus and related microorganisms are recognized as compounds by their infrared spectra. It begins to appear that each class of mycobacteria may be characterized by the chemical content of its lipid extract. The chemical identification of the atypical strains, micro-organisms which cause a disease resembling tuberculosis, is highly desired because no adequate biological tests have yet been developed. The chemical identification of this group of organisms is being attempted, and the results will be presented.”
Description
$^{*}$Supported in part by the Faculty Research Fund, University of Michigan, The American Trudeau Society, and the Research Corporation.
Author Institution: Professor Emeritus, Physics, University of Michigan; Assistant Professor, Medical Microbiology, University of Wisconsin
Author Institution: Professor Emeritus, Physics, University of Michigan; Assistant Professor, Medical Microbiology, University of Wisconsin