Interactions Between Septins and a Protein Involved in Autophagy

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2025-05

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The Ohio State University

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Abstract

Autophagy is known as the cell’s recycling system, which allows it to collect and break down cytoplasmic components that are old, damaged, or targeted for degradation. This process is essential for cell maintenance and survival, and it can cause many ailments when dysregulated. It has been suggested that a family of conserved GTP binding proteins called septins may play a role in modulating autophagy. Through multiple assays investigating protein-protein interactions, we confirm that the core septins Cdc3 and Cdc10 do interact with the autophagy related protein Atg8. We also identify the conserved sequences in both Cdc3 and Cdc10 that allow for interaction with Atg8.

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Septins, Autophagy, Cdc3, Cdc10, Atg8

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