Automated Analysis of Dynamic Single Molecule Experiments
Loading...
Date
2018-05
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
The Ohio State University
Abstract
Single molecule total internal reflection fluorescence studies using co-localization and fluorescence resonance energy transfer have become valuable tools to study biological phenomenon. However, no comprehensive programs are publicly available to quickly analyze the videos generated from these studies. Here we present several methods to automatically analyze these videos and a user oriented graphical interface to allow researchers to easily run these analyses. For each method the algorithms used are explained and their performance is evaluated. Finally, we explain the design of the user interface and its operation.
Description
Keywords
Single Molecule, Automated, FRET, co-localization studies, TIRF