ELUCIDATING THE STRUCTURE OF SUGARS: MW SPECTROSCOPY COMBINED WITH ULTRAFAST UV LASER VAPORIZATION

Research Projects

Organizational Units

Journal Issue

Abstract

Carbohydrates are one of the most versatile biochemicalbuilding blocks, widely acting in energetic, structural or recognition processes. Even the small monosaccharides display unique structural and conformational freedom and may coexist in many open-chain or cyclic forms. We recently initiated the investigation of a series of monosaccharides using a combination of ultrafast laser vaporization and microwave spectroscopy in supersonic jet expansions. We present several structural studies on carbohydrates of aldoseso \textit{Angew. Chem. Int. Ed.} \textbf{51}, 3119-3124, 2012.} and ketoseso \textit{J. Am. Chem. Soc.} \textbf{135}, 2845-2852, 2013.} of five and six carbon sugars vaporized by UV ultrafast laser vaporization and stabilized in a jet expansion. The experimental evidence confirms that sugars exhibits a $\alpha$-/$\beta$-pyranose conformation (6-membered ring), sharply contrasting with the furanose form (5-membered ring) found in the nature (as component of RNA, sucrose). In addition, thanks to the use of enriched samples, we have experimentally determined the substitution and effective structures. Finally, the structure of several monosaccharides was compared and common structural patterns of their conformational landscape will be showed.

Description

Author Institution: Departamento de Quimica Fisica, Facultad de Ciencia y Tecnologia, Universidad del Pais Vasco (UPV-EHU), Ap. 644, E-48080 Bilbao, Spain; Departamento de Quimica-Fisica y Quimica Inorganica, Facultad de Ciencias, Universidad de Valladolid, E-47011 Spain; Institut fur Physikalische Chemie, Lehrgebiet A, Universitat Hannover, Callinstra\ss e. 3A, D-30167 Hannover, Germany; Laboratoire Analyse et Modelisation pour la Biologie et l'Environnement, Universite d'Evry val d'Essonne, F-91025 Evry; (France)

Keywords

Citation