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dc.contributor.advisorMacKay, Allison
dc.creatorHurd, Maycee
dc.description.abstractHarmful algal blooms are made up of cyanobacteria that can release microcystins (MC's) into drinking water sources, which are toxic to humans. MC's can be removed during drinking water treatment using permanganate pre-oxidation. While permanganate is effective for removing microcystins, it is also non-specific and has the ability to react with dissolved organic matter (DOM) and cyanobacterial cells that exist in the water. Permanganate's reactions with DOM and cells cause competition for MC-LR removal. Additionally, reactions with cells can cause them to lyse and release intracellular MC's into the water. Preliminary work within our group suggests that a sequential dosing technique of permanganate pre-oxidation can reduce competition by DOM. Sequential refers to the additional of fractional doses of permanganate over time as opposed to a single large dose. Kinetic models fitted to observational data of sequential dosing and singular dosing found that competition by DOM decreased with each sequential dose. In the presence of microbial DOM, sequential dosing increased MCLR removal and in the presence of terrestrial DOM sequential dosing decreased removal. A propidium Iodide (PI) staining assay was developed in order to quantify cell lysis cyanobacterial cells. This assay was validated with the Bioluminescence Assay using Vibrio fischeri. When the PI assay was used to quantify cell lysis of Microcystis cells, no significant cell lysis was observed using 10 ppm of potassium permanganate. Because of this, sequential dosing was tested using V. fischeri cells instead. Sequential dosing of permanganate in the presence of V. fischeri cells saw similar amounts of cell lysis when compared to a single dose of permanganate. This suggests that the rate of competition caused by cells does not change between doses of permanganate, unlike in the presence of DOM. The removal of MCLR by permanganate in the presence of both DOM and V. fischeri cells was estimated using a kinetic model. In only the presence of 5 mg/L of microbial DOM and 100 nM of MC, we predict one dose of 12 µM permanganate to remove 92% of MCLR after 40 minutes and two doses of 6 µM to remove 99% of MCLR after 80 minutes. After adding competition by V. fischeri cells (optical density = 0.44), removal by one and two doses of permanganate was predicted to decrease to 50% and 60% total MCLR removal, respectively. Our simulation suggests that the presence of both cells and DOM have the ability to significantly impact MCLR removal in the worst-case scenario. However, sequential dosing was expected to increase MCLR removal by 10%. This simulation assumed that the cells present would be V. fischeri, which is not representative of an actual cyanobacterial bloom. Additional work should be done to more carefully examine the cyanobacterial cells interactions with permanganate.en_US
dc.description.sponsorshipOhio Water Development Authorityen_US
dc.description.sponsorshipOhio State University College of Engineeringen_US
dc.publisherThe Ohio State Universityen_US
dc.relation.ispartofseriesThe Ohio State University. Department of Civil, Environmental and Geodetic Engineering Honors Theses; 2021en_US
dc.subjectHarmful Algal Bloomsen_US
dc.subjectDrinking Wateren_US
dc.subjectWater Treatmenten_US
dc.subjectAlgal Toxinsen_US
dc.subjectCell Lysisen_US
dc.subjectPropidium Iodideen_US
dc.titleSequential Dosing of Potassium Permanganate for Removal of Microcystin-LR During Pre-Oxidationen_US
dc.description.embargoNo embargoen_US
dc.description.academicmajorAcademic Major: Environmental Engineeringen_US

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