Optimizing Actuation of Assembly of DNA Origami Nano Structures
Keywords:optimization for DNA origami assembly
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Publisher:The Ohio State University
Series/Report no.:The Ohio State University. Department of Mechanical and Aerospace Engineering Honors Theses; 2021
DNA origami as an advanced nanotechnology has been useful in providing precise design and construction for nanostructures. Combining with different actuation mechanisms such as toehold mediated strand displacement (TMSD) or magnetic actuation can realize precise control in planar or spatial motion for nanoscale structures. Recent efforts have expanded DNA origami to micrometer scale nanostructures by integrating many nanocomponents into larger assemblies, and a key goal of this work is to achieve real-time multiplexing actuation over these micronscale assemblies. Firstly, focusing on a stiff micro-scale DNA lever assembly, this work has tested different assay conditions including varying concentrations, incubation time and purification parameters to optimize the yield of individual DNA origami structure sub-units as a basis for higher order lever assembly. Also this work aims to maximize yield of at least 1-2 micrometer polymerized nanostructures, which represents a key step for practical multiplexing. Secondly, moving towards assemblies with complex reconfiguration capabilities, a verification of reconfigurability and complex motion for a second structure system was conducted. The second system consists of a DNA origami structure comprised of 6-bars connected into a closed loop. The structure can be reconfigured into several different shapes including a rectangle, triangle, hexagon, and flat closed shape. Broadly speaking, this work has integrated optimized construction for a magnetic actuated nanopolymer and verification of the feasibility of reconfiguration for a nanostructure with high order degree of freedoms. This work expands the possibilities for complex design and practical construction at micrometer scale which enables real-time control over DNA origami structures with complex reconfigurtion.
Academic Major: Mechanical Engineering
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