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dc.contributor.advisorGunn, John
dc.creatorMoshiri, Jasmine
dc.date.accessioned2020-01-22T20:59:06Z
dc.date.available2020-01-22T20:59:06Z
dc.date.issued2016-12
dc.identifier.urihttp://hdl.handle.net/1811/90827
dc.descriptionFirst Place, Denman Undergraduate Research Forumen_US
dc.description.abstractSalmonella enterica serovar Typhi (S. Typhi), the causative agent of Typhoid Fever in humans, persists in the gallbladder of 3-5% of hosts after resolution of acute infection. In this chronic carrier state, S. Typhi forms biofilms on the surface of gallstones. This study utilized a screen of 90 kinase inhibitor compounds for in vitro anti-biofilm activity against a model organism, Salmonella enterica serovar Typhimurium (S. Typhimurium), and further characterized a promising lead: T315. T315 has anti-cancer properties and has been demonstrated to disrupt the PI3K/AKT pathway, which contributes to cancer progression. The effects of T315 on S. Typhimurium biofilm formation were analyzed using rapid attachment, viability, EC50, and biofilm dispersion assays. The compound does not exhibit bactericidal activity against S. Typhimurium, yet inhibits biofilm formation by 59.4 percent, with an EC50 of 4.61 μM. Delaying drug administration permitted assessment of biofilm inhibitory activity at varying timepoints and showed that the compound interferes specifically with the earliest stages of bacterial surface attachment. T315 is being derivatized and these compounds will be screened to identify those with a lower EC50. Additionally, we are identifying the bacterial target of this compound via direct pull-down with T315 that has been biotinylated using click-chemistry. The discovery and characterization of compounds with the ability to specifically inhibit early events in bacterial attachment will aid future studies of Salmonella biofilm formation and is a promising step towards eradication of the Salmonella chronic carriage state.en_US
dc.description.sponsorshipNational Institutes of Healthen_US
dc.description.sponsorshipSOLAR Research Funden_US
dc.description.sponsorshipOSU Undergraduate Education Summer Research Fellowship Programen_US
dc.description.sponsorshipOSU Biofilms in Human Medicine Groupen_US
dc.description.sponsorshipOSU Public Health Preparedness for Infectious Diseaseen_US
dc.description.sponsorshipOSU Dean's Discovery Programen_US
dc.language.isoen_USen_US
dc.publisherThe Ohio State Universityen_US
dc.relation.ispartofseriesThe Ohio State University. Department of Microbiology Undergraduate Research Theses; 2016en_US
dc.subjectbiofilmen_US
dc.subjectpathogenesisen_US
dc.subjectbacteriaen_US
dc.subjectsalmonellaen_US
dc.titleIdentification and Characterization of Compounds with Inhibitory Properties towards Salmonella enterica Biofilmsen_US
dc.typeThesisen_US
dc.description.embargoA three-year embargo was granted for this item.en_US
dc.description.academicmajorAcademic Major: Microbiologyen_US


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