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dc.contributor.advisorRuiz, Natividad
dc.creatorMendoza, Alicia
dc.date.accessioned2019-04-22T20:15:02Z
dc.date.available2019-04-22T20:15:02Z
dc.date.issued2019-05
dc.identifier.urihttp://hdl.handle.net/1811/87522
dc.description.abstractBiosynthesis of the bacterial peptidoglycan cell wall is essential for determining bacterial shape and protection from differences in osmolaric pressure. One necessary step for the construction of the bacterial cell wall is the translocation of the lipid-linked peptidoglycan precursor, Lipid II, across the cytoplasmic membrane. MurJ is the flippase that translocates lipid II in Escherichia coli anda target of recently discovered antibacterials. Little is known about the mechanism of flippase proteins such as MurJ. However, crystal structures recently have shown MurJ in multiple inward- and outward-open conformations. These crystal structures, along with structure-guided in vivo cysteine cross-linking data, have suggested an alternating-access mechanism of function for MurJ. Since this cross-linking method allows probing of the different conformations of MurJ in vivo, here, we utilized this method to examine how changes to residues critical to MurJ function affect the conformation of the flippase in vivo. Our data suggest that MurJ mutant proteins containing R18E stall or slow down function so they are mostly found in an inward-open conformation, while those containing R24E may slightly favor the outward-open conformation.en_US
dc.language.isoen_USen_US
dc.publisherThe Ohio State Universityen_US
dc.relation.ispartofseriesThe Ohio State University. Department of Microbiology Undergraduate Research Theses; 2019en_US
dc.subjectEscherichia colien_US
dc.subjectMurJen_US
dc.subjectbacteriaen_US
dc.subjectpeptidoglycanen_US
dc.subjectmembraneen_US
dc.titleExploring the Conformations of Nonfunctional Variants of the Lipid II Flippase MurJ in Escherichia coli.en_US
dc.typeThesisen_US
dc.description.embargoNo embargoen_US
dc.description.academicmajorAcademic Major: Molecular Geneticsen_US


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