Mapping the Regulatory Role of the HD-ZIPIII START Domain Across Plant Evolution
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Publisher:The Ohio State University
Series/Report no.:The Ohio State University. Department of Molecular Genetics Undergraduate Research Theses; 2019
To determine if the regulatory function of the START domain has been conserved across the plant kingdom, a diverged CLASS III HOMEODOMAIN-LEUCINE ZIPPER (HD-ZIP III) protein from Klebsormidium flaccidum was transformed into Arabidopsis thaliana to observe misexpression gain-of-function phenotypes. However, observation of no misexpression phenotype coupled with confocal imaging of confirmed accumulation and nuclear localization of the protein suggests Klebsormidium HD-ZIP III is non-functional in Arabidopsis. Three potential explanations are explored, and a new experiment is proposed to circumvent this short-coming.
Academic Major: Molecular Genetics
OSU Department of Molecular Genetics Wagner Research Scholarship
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