Show simple item record

dc.contributor.advisorYoder, Ryan
dc.creatorPelfrey, Ian
dc.date.accessioned2018-04-20T13:27:12Z
dc.date.available2018-04-20T13:27:12Z
dc.date.issued2018-05
dc.identifier.urihttp://hdl.handle.net/1811/84713
dc.description.abstractOrganophosphorus compounds (OPs) such as sarin, soman, and tabun are toxic nerve agents used in chemical warfare and as pesticides. These OPs covalently bond with Ser203, a catalytic residue in the enzyme acetylcholinesterase (AChE), preventing hydrolysis of the neurotransmitter acetylcholine into acetate and choline. Once exposed to an OP compound, the inhibited AChE will undergo an irreversible process known as aging, where the OP-AChE complex will dealkylate and form a stable phosphonate anion on the Ser203 residue, inactivating the enzyme. Without functioning AChE, acetylcholine accumulates in the central nervous system causing seizures, vomiting, and often death. Currently, there are no known therapeutic methods to reverse this aging process to regain enzymatic activity. However, inhibited AChE can be restored to the active AChE before the onset of the aging process by treatment with pharmaceuticals containing an oxime functional group. The goal of this project is to discover a compound that will realkylate the phosphonate group on the Ser203 in aged-AChE, which can then be restored to the active AChE by oxime treatment. Literature shows that quinone methides (QMs) are capable of alkylating phosphodiesters, which are structurally similar to the phosphylated Ser203 residue in the aged-AChE active site. Through computational methods (molecular docking, molecular dynamics, and tomodock), potential poses in AChE of a variety of quinone methide precursors (QMPs) were analyzed in silico to determine their putative efficacy in vitro.en_US
dc.language.isoen_USen_US
dc.publisherThe Ohio State Universityen_US
dc.relation.ispartofseriesThe Ohio State University. Department of Chemistry and Biochemistry Undergraduate Research Theses; 2018en_US
dc.subjectOrganophsophateen_US
dc.subjectNerve Agenten_US
dc.subjectComputationalen_US
dc.subjectQuinone Methideen_US
dc.subjectDockingen_US
dc.subjectMolecular Dynamicsen_US
dc.titleComputational Studies on Realkylation Reactions of Aged-Acetylcholinesterase with Quinone Methide Precursors for Regenerating Nerve Agent Aged Acetylcholinesteraseen_US
dc.typeThesisen_US
dc.description.embargoNo embargoen_US
dc.description.academicmajorAcademic Major: Biochemistryen_US


Files in this item

Thumbnail

Items in Knowledge Bank are protected by copyright, with all rights reserved, unless otherwise indicated.

This item appears in the following Collection(s)

Show simple item record