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dc.contributor.advisorMitchell, Shaneice
dc.creatorAsmelash, Yerdanos
dc.description.abstractAcute Myeloid Leukemia (AML) is a cancer characterized by abnormal cell growth of immature myeloid cells. Currently, AML is treatable in about 35 to 40% of adult patients who are 60 years or younger, and less favorable in patients who are 60 years or older where rates are 5 to 15%. In addition, most patients have multiple malignant clones of leukemic stem cells, each differing in their responses to treatment. However, studies have shown that inhibition of the enzyme nicotinamide phosphoribosyltransferase (NAMPT) is a possible avenue for treatment of AML. NAMPT is responsible for the production of NAD+, a key metabolite needed for various cellular functions. By inhibiting NAMPT, NAD+ production is expected to decrease, thus resulting in cell death. The purpose of this study is to evaluate the ability of KPT-9274, a novel NAMPT inhibitor, to eradicate leukemic colonies in AML. To assess the ability of KPT-9274 to decrease colony formation, three AML leukemic patient cells were treated with KPT-9274. Cells were plated and incubated on a 6-well cell culture plate, along with a control group in a semi-solid medium. After two weeks, leukemic cell colonies were counted using an inverted light microscope. The cells were washed with 20% RPMI media, underwent a dilution process to achieve a desired amount of cells, and then re-plated to assess self-renewal capacity. Colony formation decreased in the three AML leukemic patient cells by 39.7 to 75.9%. These results are consistent with previous findings that support the drug’s potential to decrease NAD+ production, leading to decrease in colony formation. Self-renewal capacity of the three patients is currently being assessed. Overall, this study provides evidence that the NAMPT inhibitor, KPT-9274, is able to eradicate malignant clones found in AML patients that may lead to patient relapse.en_US
dc.description.sponsorshipR35 CA197734en_US
dc.description.sponsorshipPelotonia Idea Awarden_US
dc.description.sponsorshipD Warren Brown Foundationen_US
dc.description.sponsorshipKaryopharm Therapeuticsen_US
dc.relation.ispartofseries2017 Fall Undergraduate Research Student Poster Forum. 11then_US
dc.subjectNAMPT inhibitor Acute Myeloid Leukemiaen_US
dc.subjectKPT-9274 alternative treatmenten_US
dc.titleNAMPT inhibitor KPT-9274 as an alternative treatment for Acute Myeloid Leukemiaen_US
dc.description.academicmajorAcademic Major: Biologyen_US

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