Taggable Pyrrolysine Analogs
Advisor:Chan, Michael K.
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Publisher:The Ohio State University
Series/Report no.:The Ohio State University. Department of Chemistry Honors Theses; 2009
N6-(1,3-dioxolancarbonyl)lysine was synthesized and incorporated into full-length mCherry and alpha synuclein proteins by reading through a UAG codon. Mass spectrometry analysis confirmed that the analog incoporates into alpha synuclein in the acetal form. Two additional pyrrolysine analogs, N6-(2-oxo-2H-chromene-3-oyl)lysine trifluoroacetate and N6-(aminoxyacetoyl)lysine ditrifluoroacetate, were synthesized but neither compound reads throuh the UAG codon.
The Ohio State University
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