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dc.contributor.advisorLee, Stephen
dc.contributor.advisorHadley, Gregg
dc.contributor.advisorLu, Wu
dc.contributor.advisorVon Visger, Jon
dc.contributor.advisorBrillson, Leonard
dc.creatorCrawford, Bridget
dc.creatorTheiss, Andrew
dc.creatorWang, Yuji
dc.creatorCasal, Patricia
dc.descriptionEngineering: 3rd Place (The Ohio State University Denman Undergraduate Research Forum)en_US
dc.description.abstractField effect transistors (FETs) are solid-state electrical devices with semiconductor channels through which charge carriers migrate and generate current. The application of an electric field proximal to the conductive channel causes a change in current depending on the sign and magnitude of the field. FETs can be modified for protein sensing by deployment of antibodies as receptors on the channel surface to create an immunologically modified FET (immunoFET). Binding of analytes brings a layer of charge proximal to the channel surface, causing modulation of current that is easily detectable, allowing for quantitative detection of unlabeled analytes. We present the successful detection of​ inflammatory chemokine CXCL9 in both murine serum and human urine from transplant patients using immunoFETs modified with anti-CXCL9 IgG. CXCL9 was detected in renal transplant urine at biologically relevant levels and correlated with rejection by renal biopsy. The presented work demonstrates the feasibility of immunoFET sensor operation in physiologic buffers, and shows the potential to provide real-time quantification and monitoring of inflammatory mediators, allowing for minimally invasive interrogation of graft status. The FET design may be scalable to allow for real-time, label-free, point-of-care diagnostic use.en_US
dc.relation.ispartofseries2014 Richard J. and Martha D. Denman Undergraduate Research Forum. 19then_US
dc.subjectprotein detectionen_US
dc.titleImmunologically Modified FETs for Protein Detection in Biological Fluidsen_US
dc.description.academicmajorAcademic Major: Biomedical Engineeringen_US
dc.description.academicmajorAcademic Major: Electrical and Computer Engineeringen_US

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