Amino Acids in the Haemolymph of Smaller European Elm Bark Beetle Larvae, Scolytus Multistriatus (Marsham) (Coleoptera: Scolytidae)

Abstract

Nineteen amino acids and two amides were detected in the haemolymph of last-instar Scolytus multistriatus larvae by thin-layer chromatography. Glycine, arginine, glutamine, lysine, ornithine, histidine, asparagine, glutamic acid, serine, proline, alanine, valine, isoleucine, and leucine were readily detected. Taurine, phenylalanine, tyrosine, a- and B- amino butyric acid, and aspartic acid were less readily detected on some chromatograms, and at times were apparently absent in the haemolymph extract. Cysteine, cystine, and cysteic acid were not adequately separated by any of the methods tried, but their presence was confirmed through co-chromatography.