Alkaline Phosphatase in the Digestive System of the Desert Locust, Schistocerca Gregaria (Forskal)
MetadataShow full item record
Citation:The Ohio Journal of Science. v69 n3 (May, 1969), 183-191
Alkaline phosphatase activity was determined in the digestive system of the desert locust, Schistocerca gregaria (Forskal), using ^-nitrophenylphosphate disodium as the substrate. The enzyme has an optimum pH of 7.4 and a linear relationship between its concentration and activity. The Michaelis constant was found to be 1.4 x 10~4M. The enzyme showed a zero order of kinetics, with an incubation period of up to 25 minutes at an optimum temperature of 40°C. The temperature coefficient (Qio) was 1.960 between 5-15°C and 10-20°C, 1.900 between 20-30°C and 25-35°C, and 1.850 between 30 and 40°C. Salt solutions of MnCl2, MgCl2, CuCl2, and CoCl2 activated the enzyme, while Na2HAsO4, Na2HPO4, CuSO4, FeCl2, and CdCl2 inhibited the activity. Maximum activation was produced by 0.01 M MnCl2.
Rights:Reproduction of articles for non-commercial educational or research use granted without request if credit to The Ohio State University and The Ohio Academy of Science is given.
Items in Knowledge Bank are protected by copyright, with all rights reserved, unless otherwise indicated.