Arsenic Release Batch Test for Sulfate-Reducing Bacteria
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Publisher:The Ohio State University
Series/Report no.:The Ohio State University. Department of Civil, Environmental and Geodetic Engineering Undergraduate Research Theses; 2013
The United States Environmental Protection Agency revised regulations related to the drinking water content of arsenic (As) in 2006 lowering the Maximum Contaminant Level (MCL) from 50 μg/L to10 μg/L. The concentration of arsenic in ground water is associated with iron-reducing, sulfate-reducing and methanogenic conditions. In highly reducing systems, the dominant pathway for arsenic release is poorly understood, but reflects contributions from sulfide minerals (e.g., pyrite) and acid-volatile monosulfides (AVS). This research will evaluate arsenic release and sequestration processes under highly-reducing conditions, with the goal of identifying mechanisms and pathways responsible for arsenic release from aquifer solids under such conditions using a single culture of sulfate-reducing organisms (insert name). During the 4 week anaerobic incubation, sampling was conducted over 20 days. Samples were analyzed to determine dissolved concentrations of inorganic elements (e.g. As, S,), and concentration of anion such as acetate. Based on the first 20 days data, the result shows that the iron started being reduced, but sulfate reduction still cannot be clearly observed due to the slow rate of anaerobic bacteria growth. This suggests, more time of sampling and analytical experiments are still needed to be conducted in the following two months.
Related Item:Academic Major: Environmental Engineering
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