Enterovirus Plaque Technique : Utilization of Maintenance Medium on Agar Overlay without Neutral Red
Creators:Frey, James R.
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Citation:The Ohio Journal of Science. v66 n5 (September, 1966), 523-527
A plaque technique for quantitating enteric viruses using liquid growth medium on the agar overlay was developed. Deletion of neutral red and staining of plaques using crystal violet, following plaque development, facilitated the technique. This method utilized a minimal number of monkey kidney epithelial cells for testing the greatest number of samples.
Author Institution: The Defiance College, Defiance, Ohio
Rights:Reproduction of articles for non-commercial educational or research use granted without request if credit to The Ohio State University and The Ohio Academy of Science is given.
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