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dc.contributor.advisorLower, Steven
dc.creatorDiBartola, Alex
dc.date.accessioned2012-06-03T20:38:39Z
dc.date.available2012-06-03T20:38:39Z
dc.date.issued2012-06
dc.identifier.urihttp://hdl.handle.net/1811/51946
dc.description.abstractStaphylococcus aureus bacterial infection is a serious problem in medicine. The initial and necessary step in virtually all S. aureus infections is binding of a bacterium to surfaces within a host (e.g., tissue or indwelling device). One of the most important binding reactions is between human fibronectin (e.g., presented on endothelial cells or coatings on prosthetic heart values) and fibronectin-binding proteins produced on the surface of S. aureus. I propose that S. aureus isolates that exhibit a clinically complicated progression of infection will show stronger and more frequent binding to fibronectin-coated surfaces in vitro. This study used methicillin- resistant S. aureus (MRSA) isolates from two distinct clinical populations: patients whose bacterial infection resolved within 4 days of antibiotic treatment (n = 7; resolving bacteremia; RB), and patients who still had positive MRSA blood cultures after ≥7 days of receiving antibiotics (n = 7; persistent bacteremia; PB). Fibronectin binding was experimentally measured by using atomic force microscopy (AFM) to probe attractive forces between a fibronectin-coated AFM tip and putative fibronectin-binding receptors on S. aureus cells from each of the 14 isolates. The bacterial isolates that originated from patients that had PB showed statistically higher binding frequency (p = 0.0218), force of binding (p <0.0001), and energy of binding (p <0.0001) relative to RB isolates. These measurements suggest that binding to fibronectin is associated with increased risk of serious infection by S. aureus.en_US
dc.language.isoen_USen_US
dc.publisherThe Ohio State Universityen_US
dc.relation.ispartofseriesThe Ohio State University. Department of Microbiology Honors Theses; 2012en_US
dc.subjectFibronectin Staphylococcus aureus MRSAen_US
dc.titleFibronectin binding of persistent versus resolving methicillin-resistant Staphylococcus aureus bacteremia isolatesen_US
dc.typeThesisen_US
dc.description.embargoNo embargoen_US


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