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dc.contributor.advisorMagliery, Thomas J.
dc.creatorSen, Shiladitya
dc.date.accessioned2012-04-24T17:22:07Z
dc.date.available2012-04-24T17:22:07Z
dc.date.issued2012-02
dc.identifier.urihttp://hdl.handle.net/1811/51768
dc.descriptionMathematical and Physical Sciences: 3rd Place (The Ohio State University Edward F. Hayes Graduate Research Forum)en_US
dc.description.abstractThe inability to accurately predict the relationship between protein’s primary sequence and its overall stability presents a major hurdle in determining the effects of mutations on protein folding. To study the effects of mutation, combinatorial libraries that involve randomizing many selected positions of a protein simultaneously, offer a broader, statistically relevant dataset. In this study, we have analyzed this complicated sequence-stability relationship using rigorous high-throughput (HT) methods. Our studies focus on the four-helix model bundle protein Rop through the means of combinatorial repacking the loop. The loop was randomized with and without an additional residue to make it more flexible. Using a novel screen we identified functional variants of Rop that were of similar or higher in stability than the parent scaffold. HT biophysical characterization and detailed Gibbs-Helmholtz studies were carried out to probe the entropy and enthalphy contribution on stability for selected variants. A potential salt bridge interaction was necessary to enhance the stability of the protein without affecting its function or fold.en_US
dc.language.isoen_USen_US
dc.relation.ispartofseries2012. Edward F. Hayes Graduate Research Forum. 26then_US
dc.subjectCombinatorialen_US
dc.subjectHigh-throughputen_US
dc.subjectStabilityen_US
dc.subjectLoop mutagenesisen_US
dc.subjectGibbs-Helmholtzen_US
dc.subjectprotein foldingen_US
dc.titleCombinatorial library approach to decipher the stability effects of loop mutagenesis in model protein scaffold via high throughput methodsen_US
dc.typeArticleen_US
dc.description.embargoA five-year embargo was granted for this item.en_US


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