Ploidy of Some Non-Spherical Nuclei in Cockroaches Calculate from Photometrically Measured DNA Content
Creators:Wilson, Louis F.
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Citation:The Ohio Journal of Science. v65 n4 (July, 1965), 212-218
A modified photometric technique for measuring the DNA content in nuclei was devised to estimate the degree of ploidy in the large flattened (oblate spheroidal) nuclei of the male accessory gland in the American cockroach, F eriplaneta americana (L.), and the German cockroach, Plattella germanica (L.). This technique involves measuring diameters of the nuclei as well as light transmission through the nuclei in two dimensions rather than one as with spherical nuclei. The measurements are then combined to form an "average nucleus." Regular photometric methods were employed on the haploid spermatid used as the standard for comparison. The modified technique produced DNA values that were nearly perfect multiples of the standard. The calculations revealed 16-ploid and 32-ploid nuclei in P. americana and 8-ploid and 16-ploid nuclei in B. germanica.
Author Institution: Lake States Forest Experiment Station, Forest Service, U.S. Department of Agriculture, St. Paul, Minnesota
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