A Modified Tri-Basic-Dye Technique for Neuroglia in Autonomic Ganglia

Abstract

A specific Tri-Basic-Dye staining technique has been developed for demonstrating neuroglia nuclei in autonomic ganglia. Criteria used for development of this technique was simplicity, specificity, minimum time consumption, paraffin sections, and good photographic qualities. Modifications of a Tri-Basic-Dye procedure, originally reported by Spoerri (1948) for nerve cells and Nissl granules, have elicited a greater differentiation of neuroglia nuclei and greater intensity of their granular pattern. Neuroglia cells are readily distinguishable and well differentiated in the presence of nerve cells and Schwann cells. This technique is recommended for all investigators interested in a simple, rapid, and excellent technique for demonstrating neuroglia nuclei and their relationship to other structures in the autonomic and peripheral nervous system.