Characterization of Streptavidin-Semiconductor Interfaces for the Development of a Biosensor
Advisor:Brillson, Leonard J.
Lee, Stephen C.
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Publisher:The Ohio State University
Series/Report no.:The Ohio State University. Department of Electrical and Computer Engineering Honors Theses; 2005
The purpose of this project is to create and characterize a biosensor based upon a modified Metal Oxide Semiconductor Field Effect Transistor (MOSFET) or FET. With the metal gate of a traditional MOSFET removed to expose the underlying SiO2 interface, proteins can be applied and charge transfer due to binding events can control the sensor’s current output. Previous work has shown that such modified FETs selectively sense gases and biomaterials in dry environments. However, optimum protein binding requires a wet environment. This project examines the electronic properties of semiconductor interfaces with biological molecules in wet conditions. Advanced sensor packaging allows for an assay that can be operated in solution. Electrical tests performed in a wet microelectronic environment successfully demonstrate that devices respond to charge transfers induced by Streptavidin/Biotin-HRP binding events.
Engineering Experiment Station
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