Intraoperative Fluorescence Surgical Goggle
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Series/Report no.:2009 Edward F. Hayes Graduate Research Forum. 23rd
Background Malignant tissue resection, especially tumor resection is a great challenge to surgeons. Due to the disseminated characteristic of these tissues, the small and varied foci and blurred margins often evade surgeon’s unaided eyes. Unfortunately, surgeons have to rely mainly on preoperative images and their experience. No effective method is available that can provide real-time reference. Based on targeted fluorescence contrast agents, we are developing an intraoperative imaging system (surgical goggle) for identifying locations and margins of tumors. Method Tumor targeted MuCC49-Cy7 is injected into a female athymic nude mice. We use light at a near infrared (NIR) range (710 – 760nm) to excite the fluorophore. An ICG filter (810-875nm) is used to collect fluorescence from MuCC49-Cy7 in vivo. Also, non-fluorescence image is acquired immediately after the fluorescence image with the illumination of light at longer wavelength. Two images are processed, merged then sent to a Head Mounted Display (HMD, vision goggle) for virtual reality display. To validate the accuracy of the system, we use surgical goggle to perform mice anatomy and visualize fluorescence in vitro. Result The emission fluorescence is not detectable with bare eyes since it is in the NIR range that provides deeper penetration. However, with the help of surgical goggle, we are able to clearly identify locations and margins of tumors on the mice. At the 1st day after IV injection of MuCC49-Cy7, we can visualize dispersive fluorescence. At the 3rd day after the injection, fluorescence mainly accumulates at tumor sites. Mice anatomy validates that the merged image correctly delineate tumors. Conclusion Our surgical goggle can provide real-time, intraoperative reference during surgery. It is a robust and reliable method for segmenting and subsegmenting tumor.
Engineering: 3rd Place (The Ohio State University Edward F. Hayes Graduate Research Forum)
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