Effects of sperm irradiation and heat shock on induction of gynogenesis in muskellunge (Esox masquinongy)
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Citation:Lin, Feng; Dabrowski, Konrad "Effects of sperm irradiation and heat shock on induction of gynogenesis in muskellunge (Esox masquinongy)," Canadian Journal of Fisheries and Aquatic Sciences, v. 53, no. 9, 1996, pp. 2067-2075.
We report here the first induction of gynogenesis in esocid fish by UV irradiation and heat shock. Optimal dosage of sperm UV irradiation was in the range of 1440-2160 J/m2. Eggs fertilized with irradiated sperm at such dosages and not heat shocked yielded 100% abnormal larvae with the fertilization rate at 48.3 ± 8.4% (mean ± SD). There were no significant differences in survival to the eyed stage under the heat shock conditions tested (5-30 min post-fertilization for 5-30 min at 26-30°C). Gynogens were induced in most of the groups with hatching rates at 0.2-3.3%. Optimal conditions for gynogenetic induction of muskellunge (Esox masquinongy) were the application of 30°C for 8-10 min beginning 20 min post-fertilization, with a yield of gynogens at 5.8-6.0% (hatched normal larvae/viable eyed-stage embryos). Ploidy was identified by chromosome preparation, silver staining for nucleolar organizer regions, and flow cytometry measurement of the nuclear DNA content using eyed-stage embryos and larvae. Haploids hatched but died within 1 week.
Funding for this project was provided by the Federal Aid in Sport Fish Restoration Program (F-69-P, Fish Management in Ohio) administered jointly by the United States Fish and Wildlife Service and the Ohio Division of Wildlife.
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