Analysis of Cell Types in Continuous Cell Lines and Analysis of the Epidermal Growth Factor Receptor Pathway in Drosophila
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Publisher:The Ohio State University
Series/Report no.:The Ohio State University. Department of Molecular Genetics Honors Theses; 2009
During my research I completed work on two separate projects. First, I worked with cell lines generated from Drosophila melanogaster embryos and characterized them for specific cell type. Our lab has recently developed a new method of efficiently creating new Drosophila cell lines. With this approach, many new lines have been created, and I screened them with immunostaining for specific cell type. Out of the 24 lines examined, I found examples of nerve, blood, and epithelial cell types. The epithelial line may be useful for future analysis of the Egfr pathway through in vitro studies. Second, I analyzed the Epidermal Growth Factor Receptor (Egfr) Pathway using the Drosophila wing as a model system. Our lab has identified numerous potential targets of the pathway, including Sulfated (Sulf1), a 6-O-endosulfatase that modifies glucosamine residues in heparan-sulfated proteoglycans. The role of Sufl1 and its interactions with the Egfr pathway ligand, Vein, were analyzed genetically. In this thesis, these two projects will be treated as two separate sections.