Cell Damage due to Hydrodynamic Stress in Fluorescence Activated Cell Sorters
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Publisher:The Ohio State University
Series/Report no.:The Ohio State University. Department of Chemical and Biomolecular Engineering Honors Theses; 2009
A common technique in biological and medical research involves sorting cells using a fluorescence activated cell sorter (FACS). These sorters can achieve a great degree of purity, close to 98% , which is useful if the only necessity is the sorting itself. In many cases, the cells must be used for further analysis after being sorted; however, researches have noticed a decrease in cell viability and slowed growth after sorting. It is hypothesized that this damage is due to the hydrodynamic stress that the FACS imposes upon cells. This study will focus on the FACS Aria, while furthering the study of THP-1 and beginning analysis of a new cell line, which belongs to the same family as THP-1, the U-937 cell line. Experiments with the cell sorter will be conducted in order to gain an understanding of the cell damage and the channel in the Aria will be modeled in order to later simulate the fluid flow through the Aria. Additionally, a micro-fluidic device will be utilized in order to study the U-937 cell line’s particular response to hydrodynamic stress. This research hopes to yield a more in depth understanding into the stress that cells undergo during cell sorting, how cells respond to this stress, such as growth kinetics, cell cycle changes, and amount of cell damage, and the variability in cell sensitivity to hydrodynamic stress.
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