QUANTIFICATION OF VIRAL RNA CONCENTRATIONS IN COMMERCIALLY AVAILABLE KILLED VACCINES FOR IBDV, BY REAL TIME RT-PCR.
Contributors:Murgia, Maria V.
Yassine, Hadi M.
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Series/Report no.:Food Animal Health Research Program. Graduate student poster competition, 2009
In this study we standardized a method for viral RNA extraction from commercially available killed and live vaccines against infectious bursal disease virus (IBDV). We also used a prototype strains (STC) of know titer to prepare killed vaccine in our laboratory. We used the same live virus to develop a standard curve . We were able to show the differences in RNA content between the different vaccines.
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