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dc.contributor.advisorOttobre, Joseph
dc.creatorPaice, Shannon
dc.date.accessioned2008-12-04T21:43:40Z
dc.date.available2008-12-04T21:43:40Z
dc.date.issued2008-12
dc.identifier.urihttp://hdl.handle.net/1811/35995
dc.description.abstractSVCT1 and SVCT2 are two forms of sodium dependent vitamin C transporters within the sheep corpus luteum. A truncated version of SVCT2 has been observed in the human, and is inhibitory to SVCT1 & 2. The full nucleotide sequences of these transporters are not known in the sheep, but partial sequences are available. The objectives of the current research were to determine the existence of a truncated form of SVCT2 in the sheep, and to sequence the 3’ ends of both transport proteins. Primer sets were designed to flank the predicted deleted sequence within SVCT2. RT-PCR and gel electrophoresis procedures were run. We found the expected (approximately 800 bp) amplicon, but no 400 bp amplicon, providing no evidence of a truncated form of SVCT2 in the sheep CL. Forward primers were designed based on the partial sequences of oSVCT1 & 2, and Oligo dT 3’ Primer was used as a reverse primer. We successfully identified an 1800 bp amplicon that potentially represents the 3’ end of oSVCT2. This research could be beneficial in improving our understanding of sheep reproduction.en
dc.language.isoen_USen
dc.publisherThe Ohio State Universityen
dc.relation.ispartofseriesThe Ohio State University. Department of Animal Sciences Honors Theses; 2008en
dc.subjectVitamin Cen
dc.subjectCorpus Luteumen
dc.titleInvestigations of Sodium Dependent Vitamin C Transporters in the Ovine Corpus Luteumen
dc.typeThesisen
dc.description.embargoNo embargoen


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