Biofilm Formation by Enterococcus species of Bovine Mammary Gland and Environmental Origins
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Publisher:The Ohio State University
Series/Report no.:The Ohio State University. Department of Animal Sciences Honors Theses; 2008
In vitro biofilm formation was compared among Enterococcus species of bovine origin. One hundred-seventeen enterococcal isolates were tested. Isolates originated from aseptically collected bovine quarter milk samples and bedding samples from a single commercial dairy. Isolates from bovine quarter milk samples originated from mammary glands with clinical mastitis, cows with high somatic cell counts, and postpartum milk samples. Bacterial species tested were Enterococcus faecium (n=80), Enterococcus casseliflavus (n=28), and Enterococcus faecalis (n=9). The bacterial species significantly contributed to the ability of organisms to produce biofilm. Enterococcus faecalis biofilm assay values were greater than for either E. faecium, E. casseliflavus, or negative controls. Biofilm results did not differ among E. faecium, E. casseliflavus, or negative controls. Origin of isolates did not affect biofilm formation. Assay values were comparable among isolates of bovine mammary gland origin and those of isolates from the environment. The ability to form biofilm in vitro does not appear to a pathogenicity factor for Enterococcus species associated with bovine intramammary infections.
Second Place, Animal Science Division, College of Food, Agricultural, and Environmental Sciences Undergraduate Research Forum
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